Methods in Enzymology: Stem Cell Tools and Other Experimental Protocols
by Robert Lanza, Robert Lanza (Editor)
This is the third of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a “cookbook” and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using “conventional” and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories.
* Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering
* Presents the latest most innovative technologies
* Addresses therapeutic relevance including FDA compliance and tissue engineering
Why Does Life Exist?
Why Are You Here? A New Theory May Hold the Missing Piece
Who Are We? Experiments Suggest You’re Not Who You Think
Does Death Exist? New Theory Says ‘No’
What Happens When You Die? Evidence Suggests Time Simply Reboots
Does the Past Exist Yet? Evidence Suggests Your Past Isn’t Set in Stone
Is Death the End? Experiments Suggest You Create Time
Do You Only Live Once? Experiments Suggest Life Not One–Time Deal
Five Reasons You Won’t Die
Why You Will Always Exist
Anything Beyond the Universe? New Theory Changes Our Destiny
Are We Part of a Single Living Organism?
Have Aliens Left The Universe? Theory Predicts We’ll Follow
Are Dreams An Extension Of Physical Reality?
