» Robert Lanza,robert lanza,lanza,Dr. Robert Lanza, dr. robert lanza,Biocentrism,biocentrismRobert Lanza - BiocentrismRobert Lanza - BiocentrismRobert Lanza - BiocentrismRobert Lanza - BiocentrismRobert Lanza - BiocentrismRobert Lanza - Biocentrism

Efficient Differentiation of Functional Hepatocytes from Human Embryonic Stem Cells

By: Sadhana Agarwal, Katherine L. Holton, Robert Lanza

Differentiation of human embryonic stem cells (hESCs) to specific functional cell types can be achieved using methods that mimic in vivo embryonic developmental programs. Current protocols for generating hepatocytes from hESCs are hampered by inefficient differentiation procedures that lead to low yields and large cellular heterogeneity. We report here a robust and highly efficient process for the generation of high-purity (70%) hepatocyte cultures from hESCs that parallels sequential hepatic development in vivo. Highly enriched populations of definitive endoderm were generated from hESCs and then induced to differentiate along the hepatic lineage by the sequential addition of inducing factors implicated in physiological hepatogenesis. The differentiation process was largely uniform, with cell cultures progressively expressing increasing numbers of hepatic lineage markers, including GATA4, HNF4, alpha-fetoprotein, CD26, albumin, and alpha-1-anti-trypsin, Cyp7A1 and Cyp3A4. The hepatocytes exhibited functional hepatic characteristics such as glycogen storage, indocyanine green (ICG) uptake and release, and albumin secretion. In a mouse model of acute liver injury, the hESC-derived definitive endoderm differentiated into hepatocytes and repopulated the damaged liver. The methodology described here represents a significant step towards the efficient generation of hepatocytes for use in regenerative medicine and drug discovery.

To view the rest of this article or download it, Click Here.

Death is Only the Beginning

Picture of dog prinst in sand on beach

If death doesn’t exist, then what happened to your dog?

Robert Lanza takes the Ice Bucket Challenge

Robert Lanza Article Thumbnail Graphic

"Before I did the #icebucketchallenge, I challenged the leader of Advanced Cell Technology (ACT), Dr. Bob Lanza, to do the Ice Bucket Challenge. He did it and leading up to it he provided a quite articulate message for context (see video). Bob is one very cool guy even without ice water."
Paul Knoepfler

Entire Company takes the Ice Bucket Challenge. Click Here to See Video.

Lanza Featured in OMNI MAGAZINE’s Collector’s Edition

OMNI Magazine cover image

Omni Magazine is back. Featured story:

Building Doctor Who’s Time Machine

What if you could travel through time just like you navigate space? The journey starts here

OMNI Magazine [Read More]

Biocentrism Explored

Streaks of light in circular pattern

Rethinking Time, Space, and the Nature of the Universe.